Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria
نویسندگان
چکیده
منابع مشابه
Real-time PCR for detection and differentiation of gram-positive and gram-negative bacteria.
We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes. All tested bacteria were identified correctly, and none gave a false-positive signal with the ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2002
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.40.11.4304-4307.2002